Fmoc-Lys(Boc)-Wang Resin (100–200 Mesh)
Preloaded Resin for Fmoc-SPPS Yielding Peptides with C-Terminal Carboxylic Acid
Fmoc-Lys(Boc)-Wang Resin is a preloaded polystyrene resin functionalized with Fmoc-protected lysine, where the ε-amino group is side-chain protected with a Boc group. Built on a Wang linker, this resin is tailored for Fmoc/tBu-based solid-phase peptide synthesis (SPPS) and provides peptides with a C-terminal carboxylic acid (-COOH) after acidic cleavage.
The resin streamlines synthesis by eliminating the need to manually couple the first amino acid and ensures a high-purity, reproducible starting point for sequences incorporating lysine at the C-terminus.
Specifications
Matrix: Polystyrene cross-linked with ~1% DVB
Linker: Wang (p-alkoxybenzyl alcohol)
Preloaded Amino Acid: Fmoc-Lys(Boc)-OH
Loading: Typically 0.4–0.8 mmol/g
Particle Size: 100–200 mesh (approx. 75–150 µm)
Compatible Chemistry: Fmoc/tBu SPPS
Final Peptide Terminus: –COOH
Cleavage: Acidolytic (TFA-based)
Key Features
Preloaded with Fmoc-Lys(Boc): Saves time and ensures consistent starting point
Side-Chain Protected (Boc): Prevents undesired branching during chain elongation
Fmoc-Compatible: Ideal for stepwise SPPS using standard Fmoc deprotection cycles
Wang Linker: Provides peptides with C-terminal carboxylic acids
High Loading Accuracy: Supports reproducible and scalable peptide synthesis
Applications
Synthesis of peptides with C-terminal lysine (e.g., enzymatic substrates, receptor ligands)
Peptides requiring site-specific modification at the lysine side chain
Used in bioconjugation strategies and peptide–drug conjugates (PDCs)
Ideal for SAR studies or incorporation into therapeutic leads
Automated or manual Fmoc-based SPPS workflows
Cleavage Protocol
| Reagent | Conditions | Outcome |
|---|---|---|
| TFA (95%) + scavengers | 1–2 hours at room temperature | Fully deprotected peptide with –COOH terminus |
Standard cleavage removes the Boc group and detaches the peptide from the resin simultaneously.
Handling & Storage
Store in a dry, dark environment, under inert atmosphere if possible
Swell in DCM or DMF prior to use
Compatible with all standard Fmoc SPPS automation platforms
Handle with gloves to avoid moisture contamination
Related Resins
| Resin | C-Terminus | Preloaded Residue | Best Use |
|---|---|---|---|
| Fmoc-Lys(Boc)-Wang Resin | –COOH | Lys(Boc) | C-terminal lysine peptides |
| Fmoc-Lys(Boc)-Rink Amide Resin | –CONH₂ | Lys(Boc) | C-terminal lysine amides |
| Fmoc-Gly-Wang Resin | –COOH | Gly | Small C-terminal residues |
| Wang Resin (unloaded) | –COOH | Custom (user loads) | Tailored synthesis strategies |
Conclusion
Fmoc-Lys(Boc)-Wang Resin (100–200 mesh) is a reliable, high-purity resin ideal for the efficient synthesis of C-terminal lysine-containing peptides. It simplifies workflows, ensures high fidelity, and is compatible with modern Fmoc SPPS protocols, whether performed manually or on automated synthesizers.
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